The thriving rise of artificial intelligence (AI) technology not only expands the dimensions of knowledge presentation but also ignites students’ passion for exploring the mysteries of life, bringing new opportunities for talent cultivation in the field of biology. This study comprehensively analyzes the application status and challenges of AI in the cultivation of biological talents, and explores the direction of its future development in combination with the latest technological trends. AI technology promotes the professional development of scientific research talents, teachers, and interdisciplinary technical talents through personalized learning, intelligent resource integration, and cross-disciplinary practice. This study provides insights for promoting the deep integration of biological teaching with AI technology, fostering high-quality development of talents in the field of biology.