While promoting regional economic development, industrial parks in mountainous areas of Guizhou also face challenges such as fragile ecological environment and tight resource constraints. Therefore, based on the theory of industrial ecology and combined with the development status and environmental pollution data of industrial parks in mountainous areas of Guizhou, this paper systematically explores the ecological transformation path from three dimensions: spatial reconstruction, technological empowerment, and green policy support, providing theoretical reference and practical path for achieving regional sustainable development.